QFT Gold Testing Process

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Team ID: 
College / Administrative Unit: 
Student Affairs
Date Started: 
May 2012

The purpose of this study is to monitor the results of Quantiferon testing for TB. This test is utilized during international screening and is important for the detection of active/latent TB. False positive results can be an issue and the rate of positivity will be monitored.


Expected Results: 5-14-13

  • Reviewed sample collection technique with all laboratory staff. Samples must be shaken immediately after collection and should be drawn gray, red, purple in order.
  • Tubes are now incubated in 37 degree incubator.
  • The incubation time was reviewed and found to be acceptable. (16 -24 hours)
  • Sent staff member to ASCLS spring meeting for QFT presentation by Dr. Richard Clark.
  • Laboratory manager met with Qiagen Cellestis representative (company that makes QFT assay) to discuss concerns. It was discovered that 2 lot numbers of tubes were reported by outside labs to show increase in positive rates; however, studies by the R&D team were unable to recreate the issue. UHS lab began tracking lot numbers in LIS inventory.
  • Benchmarking was conducted with other universities.
  • In July 2012, an internal comparison study was conducted against T-spot, another interferon assay for TB. 100% agreement was found.
  • September 2012, Based upon the LIS tracking system, it was determined that these lot numbers were not in lab inventory.
Contact Person: 
Nancy Lambert
  • Vicki Dugan, Leader
  • Sharon Fong, Member
  • Janet Nelson, Member
  • Judith Novack, Member